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KMID : 0811720090130040281
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Korean Journal of Physiology & Pharmacology
2009 Volume.13 No. 4 p.281 ~ p.285
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Naringin Protects against Rotenone-induced Apoptosis in Human Neuroblastoma SH-SY5Y Cells
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Kim Hak-Jae
Yun Dong-Hwan
Chung Joo-Ho
Park Hae-Jeong
Song Jeong-Yoon
Park Hyun-Kyung
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Abstract
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Rotenone, a mitochondrial complex I inhibitor, can induce the pathological features of Parkinson’s disease (PD). In the present study, naringin, a grapefruit flavonoid, inhibited rotenone-induced cell death in human neuroblastoma SH-SY5Y cells. We assessed cell death and apoptosis by measuring mitogen- activated protein kinase (MAPKs) and caspase (CASPs) activities and by performing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Naringin also blocked rotenone-induced phosphorylation of Jun NH2-terminal protein kinase (JNK) and P38, and prevented changes in B-cell CLL/lymphoma 2 (BCL2) and BCL2-associated X protein (BAX) expression levels. In addition, naringin reduced the enzyme activity of caspase 3 and cleavages of caspase 9, poly (ADP-ribose) polymerase (PARP), and caspase 3. These results suggest that naringin has a neuroprotective effect on rotenone-induced cell death in human neuroblastoma SH-SY5Y cells.
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KEYWORD
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Apoptosis, Naringin, Parkinson’s disease, Rotenone, SH-SY5Y
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